6FYV

X-RAY STRUCTURE OF CLK4-KD(146-480)/CX-4945 AT 2.46A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	66.335, 66.335, 190.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.360 - 2.460
R-factor	0.2041
Rwork	0.203
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fyl
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.360	2.520
High resolution limit [Å]	2.460	2.460
Rmerge	0.084	0.889
Rmeas	0.087	0.924
Number of reflections	16267
<I/σ(I)>	23.9	3.4
Completeness [%]	99.6	100
Redundancy	12.9	13.3
CC(1/2)	1.000	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	30% Pentaerythritol ethoxylate (15/4 EO/OH),0.05M Ammonium Sulfate, 0.05M TRIS