6FYU

Structure of H7(A/Shanghai/2/2013) Influenza Hemagglutinin in complex SD36

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-24
Detector	MARRESEARCH
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	101.424, 128.159, 192.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.026 - 2.643
R-factor	0.1872
Rwork	0.186
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.731
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.740
High resolution limit [Å]	2.640	2.640
Rpim	0.050
Number of reflections	73520
<I/σ(I)>	13.2
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Tri-potassium citrate, 20 % PEG3350