6FYS
Structure of single domain antibody SD83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.960, 85.700, 103.780 |
| Unit cell angles | 90.00, 93.96, 90.00 |
Refinement procedure
| Resolution | 37.446 - 2.000 |
| R-factor | 0.2018 |
| Rwork | 0.201 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.878 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.030 | 0.330 |
| Number of reflections | 74300 | 7351 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 12.5 | 4.6 |
| CC(1/2) | 1.000 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M potassium formate, 14% PEG 3350 |
