6FYF
The crystal structure of EncM V135M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.99987 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 162.268, 175.164, 130.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.959 - 2.200 |
R-factor | 0.2279 |
Rwork | 0.225 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xlo |
RMSD bond length | 0.008 |
RMSD bond angle | 0.809 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.050 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.130 | 0.510 |
Number of reflections | 93709 | |
<I/σ(I)> | 11.2 | |
Completeness [%] | 99.6 | |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M HEPES-Na, 0.2M Calcium Acetate, 20% - 30% PEG 3350, pH 7.5 |