6FYC
The crystal structure of EncM L144M mutant complex with dioxygen under 15 bars O2 pressure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.99997 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 79.794, 87.358, 128.287 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.679 - 2.000 |
R-factor | 0.2415 |
Rwork | 0.240 |
R-free | 0.27220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xlo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.113 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.680 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.540 |
Number of reflections | 61351 | |
<I/σ(I)> | 21.4 | |
Completeness [%] | 100.0 | |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M HEPES-Na, 0.2M Calcium Acetate, 20% - 30% PEG 3350, pH 7.5 |