6FYB
The crystal structure of EncM L144M mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 161.993, 174.953, 130.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.960 - 1.882 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xlo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.107 | 0.570 |
| Number of reflections | 1962341 | 20077 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 99.0 | |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M HEPES-Na, 0.2M Calcium Acetate, 20% PEG 3350, pH 7.5 |
