6FXY

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Fe2+, Mn2+, UDP-Gal - Structure from long-wavelength S-SAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	2.075
Spacegroup name	C 2 2 21
Unit cell lengths	97.040, 100.105, 224.743
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	112.372 - 2.138
R-factor	0.1956
Rwork	0.194
R-free	0.23110
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.690
Data reduction software	XDS (autoPROC + STARANISO)
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.400	2.310
High resolution limit [Å]	2.138	2.138
Rmerge	0.556	2.634
Rpim	0.054	0.086
Number of reflections	67681	9637
<I/σ(I)>	18.8	2.4
Completeness [%]	93.5	78.35
Redundancy	544.3	311.4
CC(1/2)	0.998	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 0.5 mM FeCl2, 0.5 mM MnCl2, 1 mM UDP-Gal