6FXW

Structure of Leishmania infantum type B ribose 5-phosphate isomerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.912
Spacegroup name	F 2 2 2
Unit cell lengths	80.920, 83.650, 89.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 1.570
R-factor	0.14611
Rwork	0.144
R-free	0.17829
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k7o
RMSD bond length	0.024
RMSD bond angle	2.217
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.750	1.660
High resolution limit [Å]	1.570	1.570
Number of reflections	21306
<I/σ(I)>	23.2	5.09
Completeness [%]	99.6	97.8
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Hepes pH7; 2M NH4SO4