6FXS
Structure of Trypanosoma brucei type B ribose 5-phosphate isomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.912 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 97.800, 97.800, 84.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.560 - 2.010 |
R-factor | 0.16565 |
Rwork | 0.164 |
R-free | 0.19774 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k7o |
RMSD bond length | 0.021 |
RMSD bond angle | 2.102 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.560 | 2.130 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 27783 | |
<I/σ(I)> | 22.12 | 4.45 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100mM hepes pH7.5; 1.26M NH4SO4 |