6FXR

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Fe2+, Mn2+, UDP-Gal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 2 2 21
Unit cell lengths	97.801, 100.268, 224.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.901 - 2.100
R-factor	0.2186
Rwork	0.218
R-free	0.23660
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.643
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.780	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.117	1.335
Rpim	0.081	0.922
Number of reflections	63999	4484
<I/σ(I)>	11	1.2
Completeness [%]	98.8	99.7
Redundancy	5.3	5.6
CC(1/2)	0.997	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 0.5 mM FeCl2, 0.5 mM MnCl2, 1 mM UDP-Gal