6FXL
Structure of Trypanosoma cruzi type B ribose 5-phosphate isomerase (TcRpiB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 171.160, 175.350, 93.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.580 - 1.960 |
R-factor | 0.16208 |
Rwork | 0.161 |
R-free | 0.18387 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k7o |
RMSD bond length | 0.028 |
RMSD bond angle | 2.369 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.580 | 2.080 |
High resolution limit [Å] | 1.960 | 1.960 |
Number of reflections | 99426 | 15492 |
<I/σ(I)> | 26.65 | 6.17 |
Completeness [%] | 98.8 | 96 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277.15 | 100mM phosphate citrate pH4.2; 1.6M NaH2PO4/0.4M K2HPO4 |