6FXH
Human cytosolic 5'-nucleotidase II soaked with 10mM 3-Phenyl-N-(9H-purin-6-yl)benzamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 90.900, 128.200, 129.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.751 - 2.300 |
R-factor | 0.1799 |
Rwork | 0.177 |
R-free | 0.23490 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2j2c |
RMSD bond length | 0.007 |
RMSD bond angle | 0.844 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.751 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 32680 | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 96.2 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | MGSO4, TRIS AND LIGAND, PH 8.5 |