6FXF

Crystal structure of the SAM domain of murine SLy1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 41 21 2
Unit cell lengths	44.089, 44.089, 94.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.968 - 2.050
R-factor	0.2195
Rwork	0.218
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v38
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.340	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.092
Rmeas	0.096
Rpim	0.027	0.355
Number of reflections	6358	481
<I/σ(I)>	13.9	2.1
Completeness [%]	99.9	100
Redundancy	12.6	13.6
CC(1/2)	0.999	0.381

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	0.1M K2HPO4, 2.2 M (NH4)2SO4, 0.1 M NaCl, 0.1 M imidazole