6FXD
Crystal structure of MupZ from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 42.330, 80.090, 78.820 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 31.233 - 1.450 |
| R-factor | 0.1624 |
| Rwork | 0.161 |
| R-free | 0.19470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dpo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.781 |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.233 | 1.502 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.041 | |
| Rmeas | 0.049 | |
| Rpim | 0.027 | |
| Number of reflections | 45031 | |
| <I/σ(I)> | 13.21 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM amino acids II, 0.1 M MOPSO, Bis-Tris pH 6.5 and 25% w/v PEG 4000, 40% w/v 1,2,6-hexanetriol |
