6FW4

Protein-protein interactions and conformational changes : Importance of the hydrophobic cavity of TolA C-terminal domain

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AvanceIII		600
2	Bruker	AvanceIII		950
3	Bruker	AvanceIII		800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-1H NOESY
10	1	2	3D HNCO
11	1	2	3D HCCH-TOCSY
12	2	2	2D 1H-15N HSQC
13	2	2	2D 1H-15N HSQC
14	1	2	3D 1H-15N NOESY
15	1	2	3D 1H-15N TOCSY
16	1	2	3D HCCH-TOCSY
2	1	2	2D 1H-13C HSQC
3	1	2	2D 1H-13C HSQC aromatic
4	1	2	2D 1H-15N HSQC
5	1	2	2D 1H-1H TOCSY
6	1	2	2D 1H-1H COSY
7	1	2	2D 1H-13C CBCACO
8	1	2	3D CBCA(CO)NH
9	1	2	3D HNCACB

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.9	1	300
2	6.9	2500	300

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20