6FV8
Dimer structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.100, 73.600, 101.800 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 19.926 - 3.000 |
| R-factor | 0.2659 |
| Rwork | 0.265 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fv6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.631 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.712 |
| Rmeas | 0.150 | 0.840 |
| Rpim | 0.074 | 0.442 |
| Number of reflections | 23304 | 2291 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.993 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 295 | 0.1 M Tris 0.2 M ammonium sulphate 30% PEG 500 MME(v/v) |
