6FUZ

Crystal structure of the TPR domain of KLC1 in complex with the C-terminal peptide of JIP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97630
Spacegroup name	C 1 2 1
Unit cell lengths	107.473, 90.364, 51.698
Unit cell angles	90.00, 99.80, 90.00

Refinement procedure

Resolution	45.180 - 2.700
R-factor	0.234
Rwork	0.233
R-free	0.25700
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.180	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.051	1.176
Rpim	0.047	1.067
Number of reflections	13229	1747
<I/σ(I)>	11.9	1
Completeness [%]	98.6	98.5
Redundancy	3.8
CC(1/2)	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.04 M potassium phosphate monobasic, 16% PEG8000, 20% glycerol