6FUZ
Crystal structure of the TPR domain of KLC1 in complex with the C-terminal peptide of JIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97630 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.473, 90.364, 51.698 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.25700 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.051 | 1.176 |
| Rpim | 0.047 | 1.067 |
| Number of reflections | 13229 | 1747 |
| <I/σ(I)> | 11.9 | 1 |
| Completeness [%] | 98.6 | 98.5 |
| Redundancy | 3.8 | |
| CC(1/2) | 0.999 | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.04 M potassium phosphate monobasic, 16% PEG8000, 20% glycerol |
