6FUC
Structure of aminoglycoside phosphotransferase APH(3'')-Id from Streptomyces rimosus ATCC10970
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.370, 77.660, 78.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.240 - 1.170 |
| R-factor | 0.1394 |
| Rwork | 0.138 |
| R-free | 0.16870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gkh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.129 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.240 | 55.240 | 1.190 |
| High resolution limit [Å] | 1.170 | 6.390 | 1.170 |
| Rmerge | 0.185 | 0.203 | 0.391 |
| Rmeas | 0.207 | 0.224 | 0.437 |
| Rpim | 0.091 | 0.092 | 0.190 |
| Total number of observations | 475347 | ||
| Number of reflections | 98425 | 718 | 4848 |
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 99.4 | 100 | 99.4 |
| Redundancy | 4.8 | 5.3 | 5 |
| CC(1/2) | 0.966 | 0.945 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277 | 0.1M Tacsimate; 17% PEG3350; 10% sucrose. |
