6FUC
Structure of aminoglycoside phosphotransferase APH(3'')-Id from Streptomyces rimosus ATCC10970
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.370, 77.660, 78.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.240 - 1.170 |
R-factor | 0.1394 |
Rwork | 0.138 |
R-free | 0.16870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gkh |
RMSD bond length | 0.020 |
RMSD bond angle | 2.129 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.17) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.240 | 55.240 | 1.190 |
High resolution limit [Å] | 1.170 | 6.390 | 1.170 |
Rmerge | 0.185 | 0.203 | 0.391 |
Rmeas | 0.207 | 0.224 | 0.437 |
Rpim | 0.091 | 0.092 | 0.190 |
Total number of observations | 475347 | ||
Number of reflections | 98425 | 718 | 4848 |
<I/σ(I)> | 5.7 | ||
Completeness [%] | 99.4 | 100 | 99.4 |
Redundancy | 4.8 | 5.3 | 5 |
CC(1/2) | 0.966 | 0.945 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 277 | 0.1M Tacsimate; 17% PEG3350; 10% sucrose. |