6FUB
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikE with the HMA domain of Pikm-1 from rice (Oryza sativa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.023, 54.345, 48.493 |
Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
Resolution | 23.700 - 1.300 |
R-factor | 0.14296 |
Rwork | 0.141 |
R-free | 0.17854 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a6w |
RMSD bond length | 0.018 |
RMSD bond angle | 1.925 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.700 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.071 | |
Number of reflections | 38170 | |
<I/σ(I)> | 12.9 | |
Completeness [%] | 99.4 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Amino acids (0.2M L-Na-Glutamate; 0.2M Alanine (racemic); 0.2M Glycine; 0.2M Lysine HCl (racemic); 0.2M Serine (racemic)); 0.1M Buffer system 2 (1M sodium HEPES, MOPS (acid)) pH 7.5; 50% v/v Precipitant mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000) |