6FU9

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with the HMA domain of Pikm-1 from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91680
Spacegroup name	P 21 21 21
Unit cell lengths	37.090, 87.130, 103.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.040 - 1.200
R-factor	0.15025
Rwork	0.149
R-free	0.18435
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6w
RMSD bond length	0.019
RMSD bond angle	1.902
Data reduction software	xia2 (0.4.0.0)
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.040	1.230
High resolution limit [Å]	1.200	1.200
Rmerge	0.049
Number of reflections	104301
<I/σ(I)>	21.7
Completeness [%]	98.5
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.12M Ethylene glycols (0.3M Diethylene glycol; 0.3M Triethylene-glycol; 0.3M Tetraethylene glycol; 0.3M Pentaethylene glycol); 0.1M Buffer system 3 (1M Tris (base); BICINE) pH 8.5; 50% v/v Precipitant mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000)