6FTU
Structure of a Quadruplex forming sequence from D. discoideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.060, 135.750, 55.200 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 67.880 - 2.950 |
| R-factor | 0.22454 |
| Rwork | 0.218 |
| R-free | 0.28705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jrn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.880 | 3.050 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.579 | |
| Rmeas | 0.058 | 0.579 |
| Number of reflections | 10099 | 742 |
| <I/σ(I)> | 17.07 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | NaCl, MgCl2, sodium cacodylate pH 6.0, 2-methyl-2,4-pentanediol (MPD), spermine tetrahydrochloride |
