6FTS
TETR(D) N82A MUTANT IN COMPLEX WITH PEG4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-07 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 68.779, 68.779, 180.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.390 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.062 | 0.525 |
Number of reflections | 14920 | 1360 |
<I/σ(I)> | 16.1 | 2.3 |
Completeness [%] | 98.7 | 91.3 |
Redundancy | 9.55 | 6.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | Precipitant: 2M (NH4)2SO4, 6% PEG400, 0.1M Tris pH8.5. 19.65mg/ml Protein, 2mM Tetracycline, 3M MgCl2. |