6FTF
Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi at 1.09 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.846, 89.066, 45.634 |
| Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
| Resolution | 40.400 - 1.092 |
| R-factor | 0.150566354651 |
| Rwork | 0.150 |
| R-free | 0.15986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6flo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.053 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 1.131 |
| High resolution limit [Å] | 1.090 | 1.092 |
| Number of reflections | 215190 | |
| <I/σ(I)> | 11.28 | |
| Completeness [%] | 81.3 | |
| Redundancy | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | Elution buffer (Size Exclusion): 50 mM Hepes pH 7.5, 50 mM NaCl, 1 mM DMSO Crystallization buffer: 17% PEG 6.000, 0.2 M calcium acetate |
