6FTE
Crystal structure of an (R)-selective amine transaminase from Exophiala xenobiotica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.431, 148.452, 78.726 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.520 |
R-factor | 0.14062 |
Rwork | 0.138 |
R-free | 0.18589 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.909 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.565 |
High resolution limit [Å] | 1.520 | 1.525 |
Rpim | 0.059 | |
Number of reflections | 47608 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 100.0 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 2 M Ammoniumsulphate, 0.1 M Sodium acetate pH 4.7 |