6FTD
Deinococcus radiodurans BphP PAS-GAF H290T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-23 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.810, 53.030, 135.880 |
| Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
| Resolution | 47.330 - 1.400 |
| R-factor | 0.15046 |
| Rwork | 0.148 |
| R-free | 0.19816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k5b |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.551 |
| Data reduction software | XDS (March 1, 2015) |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.108 | 1.362 |
| Rmeas | 0.124 | 1.552 |
| Number of reflections | 132351 | 9607 |
| <I/σ(I)> | 7.48 | 0.93 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.25 | 4.26 |
| CC(1/2) | 0.997 | 0.336 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.95 | 297 | 67 mM Sodium acetate, 3.3 % PEG400, 1mM DTT, 30% 2-methyl-2,4-pentanediol |
