6FT2
Structure of the periplasmic binding protein LAO-Q122A in complex with arginine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.410, 58.440, 101.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.620 - 1.250 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1laf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.620 | 1.320 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 62491 | 9589 |
<I/σ(I)> | 13.27 | 1.92 |
Completeness [%] | 99.3 | 96 |
Redundancy | 8.9 | 8.5 |
CC(1/2) | 0.990 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG4000; 0.1M Mes pH6.5; 0.1M CaCl2 |