6FT2
Structure of the periplasmic binding protein LAO-Q122A in complex with arginine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.410, 58.440, 101.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.620 - 1.250 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1laf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.620 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 62491 | 9589 |
| <I/σ(I)> | 13.27 | 1.92 |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 8.9 | 8.5 |
| CC(1/2) | 0.990 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG4000; 0.1M Mes pH6.5; 0.1M CaCl2 |
