6FSU
Crystal structure of E.coli BamA beta-barrel with a C-terminal extension
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 122.737, 159.378, 55.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.840 - 2.600 |
| R-factor | 0.27052 |
| Rwork | 0.269 |
| R-free | 0.29779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n75 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.106 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.840 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.156 | 1.072 |
| Rpim | 0.111 | 0.768 |
| Number of reflections | 34607 | 4151 |
| <I/σ(I)> | 7.7 | 1.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.4 | 5.5 |
| CC(1/2) | 0.997 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22 % (w/v) PEG 400, 0.07 M NaCl, 0.05 M sodium citrate pH 4.5 |
