6FSQ

Structure of A3_bGFPD, an artificial bi-domain protein based on two different alphaRep domains : A3 and a GFP binding domain (bGFPD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.970
Spacegroup name	C 2 2 21
Unit cell lengths	66.940, 89.030, 143.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.510 - 2.790
R-factor	0.2088
Rwork	0.206
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ltj
RMSD bond length	0.010
RMSD bond angle	1.339
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.869	47.869	2.960
High resolution limit [Å]	2.790	8.250	2.790
Rmerge	0.206	0.068	1.231
Rmeas	0.214	0.071	1.283
Total number of observations	139553
Number of reflections	10938	474	1680
<I/σ(I)>	12.95	38.8	2.08
Completeness [%]	99.6	99.4	98
Redundancy	12.759	11.812	12.521
CC(1/2)	0.997	0.999	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.6M Tri-sodium citrate