6FSP
Crystal structure of APRT from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.860, 82.160, 91.390 |
| Unit cell angles | 90.00, 102.58, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.23567 |
| Rwork | 0.248 |
| R-free | 0.27118 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.754 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 25557 | |
| <I/σ(I)> | 15.24 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 296 | SA 25% |
