6FSK
F194Y mutant of the Dye-decolorizing peroxidase (DYP) from Pleurotus ostreatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2015-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976252 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.717, 117.575, 140.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.220 - 1.560 |
R-factor | 0.16386 |
Rwork | 0.162 |
R-free | 0.19885 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afv |
RMSD bond length | 0.021 |
RMSD bond angle | 2.041 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.220 | 1.640 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.065 | 0.514 |
Rmeas | 0.083 | 0.649 |
Rpim | 0.051 | 0.392 |
Number of reflections | 144926 | 20996 |
<I/σ(I)> | 12.7 | 2.3 |
Completeness [%] | 97.3 | 97.4 |
Redundancy | 4.5 | 4.6 |
CC(1/2) | 0.997 | 0.745 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.75 | 295 | 0.2M MES pH=5.75 25%PEG 2000 MME |