6FSJ
Crystal structure of TCE-treated Lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.999 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.462, 78.462, 37.503 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.836 - 1.200 |
R-factor | 0.1541 |
Rwork | 0.153 |
R-free | 0.17010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bre |
RMSD bond length | 0.006 |
RMSD bond angle | 0.866 |
Data reduction software | XDS (20180126) |
Data scaling software | XDS (20180126) |
Phasing software | PHASER ((1.12_2829)) |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.836 | 1.243 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 37081 | 3649 |
<I/σ(I)> | 13.77 | |
Completeness [%] | 99.8 | 99.54 |
Redundancy | 5.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30 mg/ml Lysozyme in 20 mM Tris pH 7.5, 200 mM NaCl 1:1 with 1.0 M LiCl, 0.1 M Na citrate pH 4.0 20 % w/v PEG 6000 Cryoprotectant: Etylene glycol 30 mM TCE |