6FSB
Influenza B/Memphis/13/03 endonuclease with I38T mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-04 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.980, 61.450, 92.630 |
Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
Resolution | 46.104 - 1.800 |
R-factor | 0.1988 |
Rwork | 0.197 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fml |
RMSD bond length | 0.011 |
RMSD bond angle | 1.043 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 39336 | 5269 |
<I/σ(I)> | 7.55 | 1.17 |
Completeness [%] | 91.3 | 92.4 |
Redundancy | 1.7 | 1.7 |
CC(1/2) | 0.996 | 0.596 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein, at 15-17 mg/ml, was incubated with 10-fold molar excess of BXA for 30 min at RT, mixtures were centrifuged at RT for 5 minutes at 12000 g, and soluble fraction was used for crystallization trials (final protein concentration 8-10 mg/ml). Mother liquor was |