6FRW

X-ray structure of the levansucrase from Erwinia tasmaniensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	128.493, 128.493, 58.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.147 - 1.520
R-factor	0.1807
Rwork	0.179
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.800
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.430	1.574
High resolution limit [Å]	1.450	1.520
Number of reflections	76050
<I/σ(I)>	14.64
Completeness [%]	99.7
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	28% Glycerol, 14% PEG 4000, 1.5mM Manganese(II) chloride tetrahydrate, 1.5 mM Cobalt(II) chloride hexahydrate , 1.5 mM Nickel(II) chloride hexahydrate, 1.5 mM Zinc acetate dihydrate