6FRV
Structure of the catalytic domain of Aspergillus niger Glucoamylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.369, 73.125, 102.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.670 - 2.300 |
| R-factor | 0.2555 |
| Rwork | 0.251 |
| R-free | 0.34330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1agm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.267 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.190 | 1.516 |
| Number of reflections | 19454 | 1870 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | PEG 3350, Hepes |
