6FRH

Crystal structure of Ssp DnaB Mini-Intein variant M86

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-08-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	34.635, 40.941, 57.906
Unit cell angles	84.37, 83.45, 65.00

Refinement procedure

Resolution	31.951 - 2.030
R-factor	0.2228
Rwork	0.222
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mi8
RMSD bond length	0.002
RMSD bond angle	0.492
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (dev_2733)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.050	37.050	2.080
High resolution limit [Å]	2.030	9.060	2.030
Rmerge	0.154	0.086	0.757
Rmeas	0.183	0.101	0.897
Rpim	0.098	0.052	0.475
Number of reflections	18028	205	1329
<I/σ(I)>	5.8
Completeness [%]	96.8	98.1	96.2
Redundancy	3.4	3.6	3.5
CC(1/2)	0.982	0.991	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	0.1 M Tris pH 7.6, 22 % PEG4000