6FR8

HA1.7 Human T-Cell Receptor specific for Influenza virus epitope PKYVKQNTLKLAT presented by Human Leukocyte Antigen HLA-DR0101

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-01-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	69.530, 49.880, 72.890
Unit cell angles	90.00, 94.26, 90.00

Refinement procedure

Resolution	69.340 - 2.380
R-factor	0.22493
Rwork	0.222
R-free	0.27844
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eh8
RMSD bond length	0.017
RMSD bond angle	1.939
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.340	2.440
High resolution limit [Å]	2.380	2.380
Rmerge	0.065	1.101
Rmeas	0.098	1.638
Number of reflections	19994	1444
<I/σ(I)>	9.9	2.1
Completeness [%]	98.8	99.6
Redundancy	3.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M KSCN,0.1M Bis-Tris, 20% PEG3350