6FR5
HA1.7 TCR Study of CDR Loop Flexibility
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.080, 81.180, 64.790 |
Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
Resolution | 40.590 - 1.370 |
R-factor | 0.16887 |
Rwork | 0.168 |
R-free | 0.19069 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6eh4 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.842 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.590 | 1.410 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.049 | 0.545 |
Rmeas | 0.057 | 0.638 |
Number of reflections | 91903 | 6599 |
<I/σ(I)> | 13.7 | 2.3 |
Completeness [%] | 98.4 | 95.8 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.998 | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M Na Citrate, 0.1M TrisHCl, 30% PEG 400 |