6FQL

Crystal structure of Danio rerio Lin41 filamin-NHL domains in complex with mab-10 3'UTR 13mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	101.490, 101.490, 108.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.300 - 2.349
R-factor	0.1739
Rwork	0.172
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fpt
RMSD bond length	0.007
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.349	2.349
Number of reflections	27367	1846
<I/σ(I)>	16
Completeness [%]	99.4	92.2
Redundancy	16.4	15.4
CC(1/2)	0.990	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	273	10% PEG 8000 20% ethylene glycol 0.1 M carboxylic acids 0.1 M MES/imidazole pH 6.5