6FQI

GluA2(flop) G724C ligand binding core dimer bound to L-Glutamate (Form B) at 2.91 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41
Unit cell lengths	50.650, 50.650, 256.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.650 - 2.910
R-factor	0.216100836964
Rwork	0.214
R-free	0.26706
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftj
RMSD bond length	0.003
RMSD bond angle	0.705
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.650	3.010
High resolution limit [Å]	2.910	2.910
Rmeas	0.121
Rpim	0.068
Number of reflections	13984
<I/σ(I)>	10.7
Completeness [%]	99.6
Redundancy	3.1
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.16 M Calcium acetate, 0.08 M Sodium Cacodylate, 14.4% (w/v) PEG 8000, 20% (v/v) Glycerol, 1 mM glutamate