6FQB
MurT/GatD peptidoglycan amidotransferase complex from Streptococcus pneumoniae R6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2014-11-28 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96500 |
| Spacegroup name | H 3 |
| Unit cell lengths | 288.430, 288.430, 115.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.19315 |
| Rwork | 0.189 |
| R-free | 0.22809 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.544 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 68725 | 11055 |
| <I/σ(I)> | 15.4 | 2.6 |
| Completeness [%] | 96.2 | 96.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293.15 | 0.2 M tri-sodium citrate pH 6.1 14% PEG 3350 4 mM NiSO4 |
