6FQA
Crystal structure of the CsuC-CsuA/B chaperone-subunit preassembly complex of the archaic chaperone-usher Csu pili of Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 94.379, 94.379, 390.745 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.189 - 2.850 |
| R-factor | 0.2002 |
| Rwork | 0.197 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fq0 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 390.745 | 46.849 | 3.000 |
| High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
| Rmerge | 0.040 | 0.866 | |
| Rmeas | 0.167 | 0.043 | 0.943 |
| Rpim | 0.058 | 0.015 | 0.364 |
| Total number of observations | 195696 | 6497 | 22672 |
| Number of reflections | 24880 | 926 | 3546 |
| <I/σ(I)> | 11.3 | 35.7 | 2 |
| Completeness [%] | 99.2 | 97.4 | 99.5 |
| Redundancy | 7.9 | 7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M sodium malonate, 15% PEG 3350 |
