6FQ1
Crystal structure of the RRM12 domain of IMP3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 30.130, 41.580, 72.490 |
Unit cell angles | 92.01, 99.91, 108.94 |
Refinement procedure
Resolution | 19.440 - 1.310 |
R-factor | 0.1419 |
Rwork | 0.140 |
R-free | 0.17450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e44 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.985 |
Data reduction software | MOSFLM (CCP4 7.0.050) |
Data scaling software | SCALA (CCP4 7.0.050) |
Phasing software | PHASER (CCP4 7.0.050) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.380 |
High resolution limit [Å] | 1.310 | 1.310 |
Rmerge | 0.044 | 0.423 |
Rmeas | 0.053 | 0.505 |
Rpim | 0.029 | 0.272 |
Number of reflections | 75254 | 10684 |
<I/σ(I)> | 12.3 | 2.8 |
Completeness [%] | 95.1 | 92.4 |
Redundancy | 3.2 | 3.2 |
CC(1/2) | 0.999 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.2 M KNO3, 20% PEG3350 |