6FPV
A llama-derived JBP1-targeting nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-15 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96600 |
| Spacegroup name | P 31 |
| Unit cell lengths | 57.680, 57.680, 64.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.950 - 1.640 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.658 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (refmac_5.8.0208) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.950 | 49.950 | 1.520 |
| High resolution limit [Å] | 1.470 | 5.690 | 1.470 |
| Rmerge | 0.051 | 0.040 | 1.382 |
| Rmeas | 0.057 | 0.044 | 1.541 |
| Rpim | 0.025 | 0.018 | 0.674 |
| Total number of observations | 210220 | ||
| Number of reflections | 40857 | 709 | 3942 |
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 99.7 | 100 | 98.4 |
| Redundancy | 5.1 | 5.5 | 5.1 |
| CC(1/2) | 0.999 | 0.997 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 277 | 0.3M Citric acid pH 3.5; 25% w/v Polyethylene glycol 3350 |
