6FPU
tRNA guanine Transglycosylase (TGT) in co-crystallized complex with 6-amino-2-((((3aS,5aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis([1,3]dioxolo)[4,5-b:4',5'-d]pyran-3a-yl)methyl)amino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.623, 64.690, 70.502 |
Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
Resolution | 44.229 - 1.360 |
R-factor | 0.1293 |
Rwork | 0.128 |
R-free | 0.15420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.007 |
RMSD bond angle | 0.947 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.230 | 1.440 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmeas | 0.053 | 0.586 |
Number of reflections | 83230 | 12915 |
<I/σ(I)> | 14.3 | 2.1 |
Completeness [%] | 96.8 | 93 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000, 100MM MES, 10%, DMSO, PH 5.5 |