6FPQ
Structure of S. pombe Mmi1 in complex with 7-mer RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-25 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 65 |
Unit cell lengths | 77.406, 77.406, 66.211 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.100 - 1.420 |
R-factor | 0.1442 |
Rwork | 0.143 |
R-free | 0.16780 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.591 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.100 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.084 | 1.160 |
Number of reflections | 42567 | 4135 |
<I/σ(I)> | 13.6 | 1.6 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7.7 | |
CC(1/2) | 0.999 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% (v/v) Ethanol, 10mM Magnesium sulfate, 0.1M Tris pH 8 |