6FPM
TetR(D) T103A mutant in complex with tetracycline and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.710, 68.710, 180.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.210 - 1.850 |
| R-factor | 0.22146 |
| Rwork | 0.219 |
| R-free | 0.26727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fpl |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.877 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.220 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.061 | 1.470 |
| Rmeas | 0.059 | |
| Number of reflections | 19026 | 1353 |
| <I/σ(I)> | 24.89 | 1.9 |
| Completeness [%] | 99.6 | 97.2 |
| Redundancy | 14.1 | 13.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M MES pH 6.5, 24% PEG 400, 70mM MgCl2. 2mM TC, 14.55mg/ml protein |
