6FPL
TETR(D) E147A MUTANT IN COMPLEX WITH TETRACYCLINE AND MAGNESIUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 68.020, 68.020, 179.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.590 - 1.602 |
R-factor | 0.18772 |
Rwork | 0.186 |
R-free | 0.22093 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2trt |
RMSD bond length | 0.018 |
RMSD bond angle | 1.837 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.590 | 1.644 |
High resolution limit [Å] | 1.602 | 1.602 |
Rmerge | 0.047 | |
Number of reflections | 28147 | 1959 |
<I/σ(I)> | 31.95 | |
Completeness [%] | 94.5 | 96 |
Redundancy | 17.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | Precipitant 0.1M Na-citrat, 0.8M Li2SO4, 0.5M (NH4)2SO4, 0.1mL Protein (10.19mg/ml, 0.1M MES pH 6.5) + 0.1mL 2mM Tc + 0.001mL 3M MgCl2 1:4 Precipitant/Protein |