6FPJ
Structure of the AMPAR GluA3 N-terminal domain bound to phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.150, 164.770, 63.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.000 - 1.960 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o21 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.715 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 164.770 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.063 | |
| Number of reflections | 81888 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Ammonium dihydrogen phosphate pH 4.6 PEG3350 |
