6FPD
AB21 protein from Agaricus bisporus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 64 |
| Unit cell lengths | 73.936, 73.936, 70.592 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.960 - 2.500 |
| R-factor | 0.24723 |
| Rwork | 0.243 |
| R-free | 0.31719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.960 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.043 | 0.070 |
| Number of reflections | 7689 | 1113 |
| <I/σ(I)> | 40.6 | 26.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.5 | 12 |
| CC(1/2) | 0.999 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 290 | 20 mM MES, 150 mM NaCl, pH 6.2 |
