6FPC
Structure of the PRO-PRO endopeptidase (PPEP-2) from Paenibacillus alvei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 43 |
| Unit cell lengths | 84.900, 84.900, 113.272 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.900 - 1.750 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20200 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.270 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.081 | 0.917 |
| Number of reflections | 80890 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 2.2 M Ammonium Sulphate 0.2 M Cadmium Sulphate, 0.01 M Zinc Chloride pH 9.0 adjusted with Ammonium hydroxide |
